Last edited by Faubei
Tuesday, May 5, 2020 | History

6 edition of Isolation and Induction of Neuronal Progenitor Cells found in the catalog.

Isolation and Induction of Neuronal Progenitor Cells

Rostock Spring School 2006 Contributions, Special Issue, Neurodegenerative Diseases 2007

by A. Rolfs

  • 354 Want to read
  • 10 Currently reading

Published by S Karger Pub .
Written in English

    Subjects:
  • Neuroscience,
  • Medical

  • The Physical Object
    FormatPaperback
    Number of Pages66
    ID Numbers
    Open LibraryOL12932022M
    ISBN 103805583303
    ISBN 109783805583305
    OCLC/WorldCa156830821

    These cells possess the characteristics of self-renewal and differentiation along all major neural lineages. Herein, the first isolation of NSPCs from the adult rhesus macaque brain and characterization of these cells based on their gene and protein expression profile, self-renewal, and ability to differentiate along an oligodendrocyte lineage Cited by: These data suggest that post-mortem human brain tissue is an important source of neural progenitor cells that will be useful for analysis of neural differentiation and for transplantation studies. Many UC-authored scholarly publications are freely available on this site because of the UC's open access by:

    The various isolation protocols, phenotypic properties, and methods for in vivo and in vitro neural differentiation of mesenchymal stem cells / marrow stromal cells (MSC), hematopoietic stem cells (HSC), multipotent adult progenitor cells (MAPCs), and umbilical cord blood stem cells (UCBSC) will . A widely used in vitro culture method, known as the Neurosphere Assay (NSA), has provided a means to retrospectively identify neural progenitor cells as well as to determine both their self-renewal capacity and their ability to generate the three primary cell types of the nervous system: neurons, astrocytes, and oligodendrocytes.

    Neural progenitor cells can be generated efficiently using STEMdiff™ Neural Induction Medium by either Embryoid body or Monolayer Culture Methods Vivian M. Lee 1, Alexandra Blak 1, Allen C. Eaves 1,2, Terry 1, and Sharon A. Louis 1. Stem cells translational medicine Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD [Miriam E van Strien, Jacqueline A Sluijs, Brent A Reynolds, Dennis A Steindler, Eleonora Aronica, Elly M Hol] PMID


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Isolation and Induction of Neuronal Progenitor Cells by A. Rolfs Download PDF EPUB FB2

Neural precursor cells are dispersed widely throughout the adult brain. A population of multipotential progenitor cells, capable of giving rise to bot Cited by: 7. The isolation and culture of neural progenitor cells (NPCs) from pluripotent stem cells has facilitated in vitro mechanistic studies of diseases related to the nervous system, as well as discovery of new medicine.

In addition, NPCs are envisioned to play a crucial role in future cell replacement by: 1. Request PDF | Isolation and induction of adult neural progenitor cells | Neural precursor cells are dispersed widely throughout the adult brain.

differentiation demonstrated that the majority of cells with neuronal morphology expressed voltage-gated so-dium and potassium currents. These data suggest that post-mortem human brain tissue is an important source of neural progenitor cells that will be useful for analysis of neural differentiation and for transplantation studies.

For immunophenotyping, cells were isolated from the postmor-tem SVZ of three donors. The cells were centrifuged, and the obtained cell pellet was taken up in NPC medium and cultured as adherent cells on laminin-coated (20 mg/ml, 3 hours at 37°C) well chamber slides (5, cells per well) and allowed to ad-here for 5 by: Recently, neural stem/progenitor cells have been identified in the cerebral cortex, as well as previously recognized regions such as the subventricular or subgranular zones of the hippocampus, suggesting that a contribution of cortex‐derived neural stem/progenitor cells Cited by:   Neural progenitor cells (NPCs) or neural stem cells are important tools for investigating central nervous system (CNS) development.

NPCs can be used in therapeutic strategies and for characterizing differentiation mechanisms. Here, we describe methods for isolating and culturing embryonic by: 3.

Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells.

The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Neural stem cells are characterized by their abilities to self-renew and to generate the different cell types found in the central nervous system.

Isolation and in vitro analyses of neural stem/progenitor cells (NSCs) have proved to be an important method for deciphering the cellular and molecular mechanisms underlying neurogenesis, and for optimizing stem cell-based treatment of neurological disorders Cited by:   Abstract.

The subcortical white matter of the adult human brain harbors a pool of glial progenitor cells. These cells can be isolated by fluorescence-activated cell sorting Cited by: The cytoarchitectonic arrangement and appearance of the neuronal progenitor cells is quite varied in the primate compared to the rodent; in some locations the cells are aligned in parallel arrays.

In vitro neuronal cultures have been used as a fundamental tool for studies of neurological diseases. Primary animal (mostly rodent) neural cultures 1,2 and human neural cell lines derived from gliomas or other tumors are the most commonly used in such studies.

However, it has been recognized that there are significant differences between rodent and human by: The isolation of multipotent neural precursors from adult human should open new perspectives to study adult neurogenesis and for brain repair.

The present study describes the in vitro isolation from adult human brains of a progenitor responsive to both epidermal and basic fibroblast growth factors that forms spheres as it by: Discovery of neural stem/progenitor cells is one of the greatest achievements in the field of neuroscience.

Recently, more and more information is being confirmed about ongoing neurogenesis in certain areas of the mammalian brain, which is provided by a pool of stem cells [1, 2].

A significant step in the study of neural progenitor cells was. We detected several neural stem cell-specific genes, such as Sox2, Notch1, Pax6, and MASH1, consistent with the view that the cells from DRG explants are likely neural progenitor cells. The expression of Wnt1 in sphere preparations suggests a role of Wnt signaling in.

Isolation of CD + /GFAPδ + neural progenitor cells derived from the subventricular zone of patients with a neurodegenerative disorder. (A): mRNA expression levels in CD + and CD − fractions directly after isolation. Data are expressed as median and interquartile ranges.

Analysis of significance was performed by Mann‐Whitney U by: Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele.

Marotta M(1), Fernández-Martín A(2), Oria M(1), Fontecha CG(2), Giné C(2), Martínez-Ibáñez V(2), Carreras E(3), Belfort Cited by: 3. Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele. 1 Find the latest peer-reviewed research articles and preprints on Coronavirus here.

Some protocols require the differentiation of cells via a embryoid body, 3D culture or neurosphere s 20 or induction of a pan-neural progenitor cell Cited by: 7. Within 1 week, migrating cells that express the early neural crest markers p75 and HNK1 as well as numerous other genes associated with neural crest induction such as SNAIL, SLUG, and SOX10 are detectable.

Fluorescence-activated cell sorting (FACS)-based isolation of the ppositive population enriches for cells with genetic, phenotypic, and Cited by:. The culture of NSCs in chemically defined neural induction medium results in the differentiation of progenitor cells into different neuronal subtypes.

The proliferative NPCs are characterized by the expression of SRY (sex determining region Y)-box 2 and paired box 6 .The addition of STEMdiff™ SMADi Neural Induction Supplement to STEMdiff™ Neural Induction Medium promotes the efficient conversion of human ES and iPS cells to CNS-type NPCs and inhibits the unwanted differentiation of non-CNS-type cells.Objective—To isolate and characterize neural stem and progenitor cell populations in the brain of adult dogs.

Animals—7 healthy adult dogs. Procedures—Dogs (age, 10 to 60 months) were euthanized for reasons unrelated to the subventricular zone (SVZ) adjacent to the lateral ventricles and subgranular zone (SGZ) of the hippocampus were isolated and used to generate single cell Cited by: